John W. Hollingsworth, MD

Associate Professor of Medicine
Assistant Professor of Immunology
Department / Division:
Medicine / Medicine-Pulmonary
DUMC 103004
Durham, NC 27710
Appointment Telephone:
Office Telephone:
  • MD, University of Texas Medical Branch School of Medicine, 1996
  • Internal Medicine, University of Texas Medical Branch, 1999
  • Pulmonary and Critical Care Medicine, Duke University Medical Center, 2004
Clinical Interests:
Critical care medicine, environmental lung disease, asthma, COPD
Research Interests:
John W. Hollingsworth MD is an Associate Professor of Medicine and an Assistant Professor of Immunology at Duke University Medical Center. Dr. Hollingsworth received his medical degree, attended residency, and served as chief resident at the University of Texas Medical Branch. He completed a fellowship in Pulmonary and Critical Care Medicine at Duke University Medical Center. The major goal of Dr. Hollingsworth’s research program is to better understand the interaction between exposure to common environmental factors and host vulnerability to complex heritable lung disease. Laboratory work has focused on using environmental exposures to better understand fundamental mechanisms that regulate airway hyperresponsiveness and innate immunity. Studies additionally focus on the impact of environmental exposures during vulnerable periods of development on prevalence of disease and the mechanisms that common exposures can alter heritable risk of complex immunological lung disease. Work focus on both genetic and epigenetic mechanisms that contribute to disease pathogenesis. His program is dedicated to understanding the role of common environmental exposures and host factors in the development of human disease.
Representative Publications:
  • Ledford, JG; Mukherjee, S; Kislan, MM; Nugent, JL; Hollingsworth, JW; Wright, JR. Surfactant protein-A suppresses eosinophil-mediated killing of Mycoplasma pneumoniae in allergic lungs. PLoS One. 2012;7:e32436.  Abstract
  • Pinkerton, KE; Rom, WN; Akpinar-Elci, M; Balmes, JR; Bayram, H; Brandli, O; Hollingsworth, JW; Kinney, PL; Margolis, HG; Martin, WJ; Sasser, EN; Smith, KR; Takaro, TK; American Thoracic Society Environmental Health Policy Committee. An official American Thoracic Society workshop report: Climate change and human health. Proceedings of the American Thoracic Society. 2012;9:3-8.  Abstract
  • Potts-Kant, EN; Li, Z; Tighe, RM; Lindsey, JY; Frush, BW; Foster, WM; Hollingsworth, JW. NAD(P)H:quinone oxidoreductase 1 protects lungs from oxidant-induced emphysema in mice. Free Radical Biology and Medicine. 2012;52:705-715.  Abstract
  • Al-Hegelan, M; Tighe, RM; Castillo, C; Hollingsworth, JW. Ambient ozone and pulmonary innate immunity. Immunologic Research: a selective reference to current research and practice. 2011;49:173-191.  Abstract
  • Frush, S; Li, Z; Potts, EN; Du, W; Eu, JP; Garantziotis, S; He, YW; Foster, WM; Hollingsworth, JW. The role of the extracellular matrix protein mindin in airway response to environmental airways injury. Environmental Health Perspectives. 2011;119:1403-1408.  Abstract
  • Heise, RL; Stober, V; Cheluvaraju, C; Hollingsworth, JW; Garantziotis, S. Mechanical stretch induces epithelial-mesenchymal transition in alveolar epithelia via hyaluronan activation of innate immunity. Journal of Biological Chemistry. 2011;286:17435-17444.  Abstract
  • Li, Z; Potts-Kant, EN; Garantziotis, S; Foster, WM; Hollingsworth, JW. Hyaluronan signaling during ozone-induced lung injury requires TLR4, MyD88, and TIRAP. PLoS One. 2011;6:e27137.  Abstract
  • Lindsey, JY; Ganguly, K; Brass, DM; Li, Z; Potts, EN; Degan, S; Chen, H; Brockway, B; Abraham, SN; Berndt, A; Stripp, BR; Foster, WM; Leikauf, GD; Schulz, H; Hollingsworth, JW. c-Kit is essential for alveolar maintenance and protection from emphysema-like disease in mice. American Journal of Respiratory and Critical Care Medicine. 2011;183:1644-1652.  Abstract
  • Sweeney, TE; Suliman, HB; Hollingsworth, JW; Welty-Wolf, KE; Piantadosi, CA. A toll-like receptor 2 pathway regulates the Ppargc1a/b metabolic co-activators in mice with Staphylococcal aureus sepsis. PLoS One. 2011;6:e25249.  Abstract
  • Tighe, RM; Li, Z; Potts, EN; Frush, S; Liu, N; Gunn, MD; Foster, WM; Noble, PW; Hollingsworth, JW. Ozone inhalation promotes CX3CR1-dependent maturation of resident lung macrophages that limit oxidative stress and inflammation. Journal of Immunology. 2011;187:4800-4808.  Abstract
  • Garantziotis, S; Li, Z; Potts, EN; Lindsey, JY; Stober, VP; Polosukhin, VV; Blackwell, TS; Schwartz, DA; Foster, WM; Hollingsworth, JW. TLR4 is necessary for hyaluronan-mediated airway hyperresponsiveness after ozone inhalation. American Journal of Respiratory and Critical Care Medicine. 2010;181:666-675.  Abstract
  • Hollingsworth, JW; Free, ME; Li, Z; Andrews, LN; Nakano, H; Cook, DN. Ozone activates pulmonary dendritic cells and promotes allergic sensitization through a Toll-like receptor 4-dependent mechanism. Journal of Allergy and Clinical Immunology. 2010;125:1167-1170.  Abstract
  • Li, Z; Potts, EN; Piantadosi, CA; Foster, WM; Hollingsworth, JW. Hyaluronan fragments contribute to the ozone-primed immune response to lipopolysaccharide. Journal of Immunology. 2010;185:6891-6898.  Abstract
  • Martin, SS; Hollingsworth, CL; Norfolk, SG; Wolfe, CR; Hollingsworth, JW. Reversible cardiac dysfunction associated with pandemic 2009 influenza A(H1N1). Chest. 2010;137:1195-1197.  Abstract
  • Norfolk, SG; Hollingsworth, CL; Wolfe, CR; Govert, JA; Que, LG; Cheifetz, IM; Hollingsworth, JW. Rescue therapy in adult and pediatric patients with pH1N1 influenza infection: a tertiary center intensive care unit experience from April to October 2009. Critical Care Medicine. 2010;38:2103-2107.  Abstract
  • Snyder, JC; Reynolds, SD; Hollingsworth, JW; Li, Z; Kaminski, N; Stripp, BR. Clara cells attenuate the inflammatory response through regulation of macrophage behavior. American Journal of Respiratory Cell and Molecular Biology. 2010;42:161-171.  Abstract
  • Sweeney, TE; Suliman, HB; Hollingsworth, JW; Piantadosi, CA. Differential regulation of the PGC family of genes in a mouse model of Staphylococcus aureus sepsis. PLoS One. 2010;5:e11606.  Abstract
  • Garantziotis, S; Li, Z; Potts, EN; Kimata, K; Zhuo, L; Morgan, DL; Savani, RC; Noble, PW; Foster, WM; Schwartz, DA; Hollingsworth, JW. Hyaluronan mediates ozone-induced airway hyperresponsiveness in mice. Journal of Biological Chemistry. 2009;284:11309-11317.  Abstract
  • Li, Z; Garantziotis, S; Jia, W; Potts, EN; Lalani, S; Liu, Z; He, YW; Foster, WM; Hollingsworth, JW. The extracellular matrix protein mindin regulates trafficking of murine eosinophils into the airspace. Journal of Leukocyte Biology. 2009;85:124-131.  Abstract
  • Reynolds, CM; Suliman, HB; Hollingsworth, JW; Welty-Wolf, KE; Carraway, MS; Piantadosi, CA. Nitric oxide synthase-2 induction optimizes cardiac mitochondrial biogenesis after endotoxemia. Free Radical Biology and Medicine. 2009;46:564-572.  Abstract
  • Boon, K; Tomfohr, JK; Bailey, NW; Garantziotis, S; Li, Z; Brass, DM; Maruoka, S; Hollingsworth, JW; Schwartz, DA. Evaluating genome-wide DNA methylation changes in mice by Methylation Specific Digital Karyotyping. BMC Genomics. 2008;9:598.  Abstract
  • Brass, DM; Hollingsworth, JW; Cinque, M; Li, Z; Potts, E; Toloza, E; Foster, WM; Schwartz, DA. Chronic LPS inhalation causes emphysema-like changes in mouse lung that are associated with apoptosis. American Journal of Respiratory Cell and Molecular Biology. 2008;39:584-590.  Abstract
  • Garantziotis, S; Zudaire, E; Trempus, CS; Hollingsworth, JW; Jiang, D; Lancaster, LH; Richardson, E; Zhuo, L; Cuttitta, F; Brown, KK; Noble, PW; Kimata, K; Schwartz, DA. Serum inter-alpha-trypsin inhibitor and matrix hyaluronan promote angiogenesis in fibrotic lung injury. American Journal of Respiratory and Critical Care Medicine. 2008;178:939-947.  Abstract
  • Hollingsworth, JW; Maruoka, S; Boon, K; Garantziotis, S; Li, Z; Tomfohr, J; Bailey, N; Potts, EN; Whitehead, G; Brass, DM; Schwartz, DA. In utero supplementation with methyl donors enhances allergic airway disease in mice. Journal of Clinical Investigation. 2008;118:3462-3469.  Abstract
  • Brass, DM; Hollingsworth, JW; Fessler, MB; Savov, JD; Maxwell, AB; Whitehead, GS; Burch, LH; Schwartz, DA. The IL-1 type 1 receptor is required for the development of LPS-induced airways disease. Journal of Allergy and Clinical Immunology. 2007;120:121-127.  Abstract
  • Garantziotis, S; Hollingsworth, JW; Ghanayem, RB; Timberlake, S; Zhuo, L; Kimata, K; Schwartz, DA. Inter-alpha-trypsin inhibitor attenuates complement activation and complement-induced lung injury. Journal of Immunology. 2007;179:4187-4192.  Abstract
  • Hollingsworth, JW; Kleeberger, SR; Foster, WM. Ozone and pulmonary innate immunity. Proceedings of the American Thoracic Society. 2007;4:240-246.  Abstract
  • Hollingsworth, JW; Li, Z; Brass, DM; Garantziotis, S; Timberlake, SH; Kim, A; Hossain, I; Savani, RC; Schwartz, DA. CD44 regulates macrophage recruitment to the lung in lipopolysaccharide-induced airway disease. American Journal of Respiratory Cell and Molecular Biology. 2007;37:248-253.  Abstract
  • Hollingsworth, JW; Maruoka, S; Li, Z; Potts, EN; Brass, DM; Garantziotis, S; Fong, A; Foster, WM; Schwartz, DA. Ambient ozone primes pulmonary innate immunity in mice. Journal of Immunology. 2007;179:4367-4375.  Abstract
  • Hollingsworth, JW; Whitehead, G; Berman, KG; Tekippe, EM; Gilmour, MI; Larkin, JE; Quackenbush, J; Schwartz, DA. Genetic basis of murine antibacterial defense to streptococcal lung infection. Immunogenetics. 2007;59:713-724.  Abstract
  • Yang, IV; Burch, LH; Steele, MP; Savov, JD; Hollingsworth, JW; McElvania-Tekippe, E; Berman, KG; Speer, MC; Sporn, TA; Brown, KK; Schwarz, MI; Schwartz, DA. Gene expression profiling of familial and sporadic interstitial pneumonia. American Journal of Respiratory and Critical Care Medicine. 2007;175:45-54.  Abstract
  • Hollingsworth, JW; Whitehead, GS; Lin, KL; Nakano, H; Gunn, MD; Schwartz, DA; Cook, DN. TLR4 signaling attenuates ongoing allergic inflammation. Journal of Immunology. 2006;176:5856-5862.  Abstract
  • Hollingsworth, JW; Chen, BJ; Brass, DM; Berman, K; Gunn, MD; Cook, DN; Schwartz, DA. The critical role of hematopoietic cells in lipopolysaccharide-induced airway inflammation. American Journal of Respiratory and Critical Care Medicine. 2005;171:806-813.  Abstract
  • Suliman, HB; Welty-Wolf, KE; Carraway, MS; Schwartz, DA; Hollingsworth, JW; Piantadosi, CA. Toll-like receptor 4 mediates mitochondrial DNA damage and biogenic responses after heat-inactivated E. coli. The FASEB Journal. 2005;19:1531-1533.  Abstract
  • Hollingsworth, JW; Cook, DN; Brass, DM; Walker, JK; Morgan, DL; Foster, WM; Schwartz, DA. The role of Toll-like receptor 4 in environmental airway injury in mice. American Journal of Respiratory and Critical Care Medicine. 2004;170:126-132.  Abstract
  • Cook, DN; Hollingsworth, JW; Schwartz, DA. Toll-like receptors and the genetics of innate immunity. Current Opinion in Allergy and Clinical Immunology. 2003;3:523-529.  Abstract
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